Suzuki Lab
McArdle Laboratory for Cancer Research

Publications and Patent

Research Highlight

A portion of our research is featured on the following websites. For more details, please visit the link below.

Publications

Graduate student/Postbac authorship is bolded. Undergraduate Student authorship is underlined. (†Equal contribution, §Lead contact, *Corresponding author, #Co-first authors)

Suzuki Google Scholar Profile        Pubmed Link            Bibliography        ORCID

2025

41) Reddy, S., Chen, YL., Suzuki, A.* “Accurate identification of cell cycle stages in RPE1 cells using the ImmunoCellCycle-ID method”,  Under review

40) Bandini, M., Ghone, D., Evans III, EL., Suzuki, A.*, Sherer, NM.* “HIV-1 Vif and Vpr cooperatively modulate the cell cycle to maximize per cell virion production”,  Under review

39) Kim, EJ., Bacabac, M.S.,  Wang, Y., M., Chen, YL., Ma, M.,  Liu, P., Zhou, J., Fry, C.J., Hoffman, J.R., Yu, M., Li, L., Suzuki, A., Li, S., Xu, W. “CARM1-mediated MAP2K4 methylation potentiates the oncogenic functions of MAP2K4 and constitutes a targetable dependency in triple-negative breast cancer”, Cancer Research, doi: 10.1158/0008-5472.CAN-24-3476., 2025

38) Chen, YC., Takada M., Nagornyuk, A., Yu, M., Yamada, H., Nagashima, T., Ohtsuka, M., DeLuca, JG., Markus, S., Takaku, M., Suzuki, A.* “Inhibition of p38-MK2 pathway enhances the efficacy of microtubule inhibitors in breast cancer cells“, eLife, 13:RP104859. 2025. Original BioRxiv version is available here.

eLife Assessment of this work: This study provides valuable findings that MK2 inhibitor CMPD1 can inhibit the growth, migration and invasion of breast cancer cells both in vitro and in vivo. The evidence supporting the claims of the authors is solid, although the detailed molecular mechanism and additional animal experiments would strengthen the paper. This study will be of interest to the breast cancer field.

37) Ghone, D., Evans III, EL.,  Bandini, M., Stephenson, KG., Sherer, NM.*, Suzuki, A.* “HIV-1 Vif disrupts phosphatase feedback regulation at the kinetochore leading to a pronounced pseudo-metaphase arrest“, eLife, 13:RP101136, 2025. Original bioRxiv is available here.

This is our first “Virology” and “HIV-1 research” paper!!

eLife Assessment of this work: This study provides a convincing explanation for why HIV-1 Vif causes a qualitatively different cell cycle arrest to its accessory gene counterpart Vpr. The authors use elegant time-dependent microscopy reporter assays in immortalized tumor cell models to show that HIV-1 Vif causes a pseudo-metaphase arrest rather than a G2 arrest. The metaphase arrest correlates with dysregulation of the kinetochore that could be explained by the loss of phosphatase functions that determine chromosome-microtubule interactions. These valuable findings lay the groundwork for additional studies examining the mechanisms and consequences of this Vif-dependent phenotype in the viral life cycle and in primary cells more relevant to HIV-1 pathogenesis.

36) Chen, YL., Reddy, S., Suzuki, A.* “Reversible and effective cell cycle synchronization method for studying stage-specific investigation“, Life Science Alliance, Mar 2025, 8 (5), e202403000. Original bioRxiv version is available here.

35) Norman, R.†, Chen, YC.†, Recchia M., Loi, J., Rosemarie, Q., Lesko, S., Patel, S., Sherer, NM., Takaku, M., Burkard, M., Suzuki, A.* “One step 4x and 12x 3D-ExM enables robust super-resolution microscopy of nanoscale cellular structures“, Journal of Cell Biology , 224 (2): e202407116, 2025. Original bioRxiv version is available here.

This paper is selected for the JCB Journal Cover!!

2024

34) Chen, YC., Kilic, E., Wang, E., Rossman, W., Suzuki, A.* “CENcyclopedia: Dynamic Landscape of Kinetochore Architecture Throughout the Cell Cycle“,  bioRxiv

This preprint is selected for “preLights” in December 2024!!

33) Chen, YL. and Suzuki, A.* “Advanced High-Resolution and Super-Resolution Microscopy Techniques for Investigating Chromatin Structure and Epigenetics”, Methods in Molecular Biology, in press

32) Chen, YL., Chen, YC., Suzuki, A.* “ImmunoCellCycle-ID: A high-precision immunofluorescence-based method for cell cycle identification“, Journal of Cell Science, 15, November 2024; 137 (22): jcs263414, Original bioRxiv version is available here.

This paper is selected for “Research Highlight” in Journal of Cell Science!!

31) Loi, J., Ghone D., Qu, X., Suzuki, A*. “3D-Aligner: An advanced computational tool designed to correct image distortion in expansion microscopy for precise 3D reconstitution and quantitative analysis“, Communications Biology, 7, 1325, (2024). Original bioRxiv version is available here.

30) Alsina, FC., Lupan, BM., Lin, LJ., Musso, CM., Mosti, F., Newman, CR., Wood, LM., Suzuki, A., Agostino, M., Moore, JK., Silver, DL. “The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton“, Cell Reports, Aug 23;43(9), 2024

29) Lin, CC. and Suzuki, A.* “Calibrating Fluorescence Microscopy with 3D-Speckler (3D fluorescence speckle analyzer)“, Bio-protocol, Vol 14, Iss 16, Aug 20, 2024

28) Yamada H., Takada M., Ghone D., Yu M.,  Nagashima, T., Fujimoto, H., Sakakibara, J., Hasegawa Y., Takao, S., Yamada, A., Narui, K., Ishikawa, T., Suzuki, A., Ohtsuka, M. “Eribulin induces micronuclei and enhances the nuclear localization of cGAS in triple-negative breast cancer cells“, Scientific Reports, 14(1):14146, 2024

27) Wu, C., Balakrishnan, VK., Parihar, PS., Konovolov, K., Chen, YC., Merrill, RA., Wei, H., Carragher, B., Sundaresan, R., Cui, Q., Wadzinski, BE., Swingle, MR., Musiyenko, A., Honkanen, R., Chung, W., Suzuki, A., Strack, S., Huang, X., Xing, Y. “Extended regulation interface coupled to the allosteric network and disease mutations in the PP2A-B56 holoenzyme“,   Proceedings of the National Academy of Sciences (PNAS), 121 (1) e2310727120, 2024

2023

26) Loi, J., Qu, X., Suzuki, A.* Semi-automated 3D fluorescence speckle analyzer (3D-Speckler) for microscope calibration and nanoscale measurement.  Journal of Cell Biology, 222, (4), 2023.

2022

25) Bakshi, DD., Chen, YC., Wuerzberger-Davis, SM., Ma, M., Waters, BJ., Li, L., Suzuki, A.,  Miyamoto, S. Ectopic CH60 mediates HAPLN1-induced cell survival signaling in multiple myeloma. Life Science Alliance, Vol 6, no 3, e202201636, (2022).

2021

24) Kim. EJ., Liu, P., Zhang, S., Donahue, K., Wang, Y., Schehr, JL., Wolfe, SK., Dickerson, A., Lu, L., Rui, L., Zhong, X., Wisinski, KB., Yu, M., Suzuki, A., Lang, JM., Ong, IM., Xu, W. BAF155 methylation drives metastasis by hijacking super-enhancers and subverting anti-tumor immunity. Nucleic Acids Research, 49 (21), (2021).

2020

23) Franks, JL, Martinez-Chacin, RC., Wang, X., Tiedemann RL., Bonacci, T., Choudhury, R., Bolhuis, DL., Enrico, TP., Mouery, RD., Damrauer, JS, Yan, F., Harrison, JS., Major, MB., Hoadley, KA.,  Suzuki, A., Rothbart, SB., Brown, NG., Emanuele, MJ. In silico APC/C substrate discovery reveals cell cycle-dependent degradation of UHRF1 and other chromatin regulators. PLoS Biology, 18 (12), e3000975, (2020).

2018

22) Suzuki, A.*, Varma, D.* Cell Division: The Unattached Kinetochore Wears an Expansive RZZ Coat. Current Biology, 28, R1250-1252, (2018).

21) Suzuki, A.†, §, *, Gupta, A.†, Long, SK., Evans, R., Badger, BL., Salmon, ED., Biggins, S.*, Bloom, K. A Kinesin-5, Cin8, Recruits Protein Phosphatase 1 to Kinetochores and Regulates Chromosome Segregation. Current Biology, 28 (17), 2697-2704.
-Featured in Dispatch entitled “Cell Division: Here Comes the Kinesin Cavalry” in Current Biology, 28 (17), R943-R946.

20) Agarwal, S., Smith, KP., Zhou, Y., Suzuki, A., McKenney RJ., Varma, D. Cdt1 stabilizes kinetochore-microtubule attachments via an Aurora B kinase-dependent mechanism. Journal of Cell Biology, 217 (10), 2697-2704, (2018).

19) Bonacci, T., Suzuki, A., Grant, GD., Stanley, N., Cook, JG., Brown, NG., Emanuele MJ. Cezanne/OTUD7B is a cell cycle-regulated deubiquitinase that antagonizes the degradation of APC/C substrates. EMBO journal, 37 (15), 1-17, (2018).

18) Fadero, TC., Gerbich, TM.†, Rana, K.†, Suzuki, A., DiSalvo, M., Schaefer, KN., Heppert, JK., Boothby, TC., Goldstein, B., Peifer, M., Allbritton, NL., Gladfelter, AS., Maddox, AS., and Maddox, PS. LITE microscopy: Tilted light-sheet excitation of model organisms offers high resolution and low photobleaching. Journal of Cell Biology, 217 (5), 1869-1882 (2018).

17) Suzuki, A.*, Long, SK., Salmon, ED. An Optimized Method for 3D Fluorescence Co-Localization Applied to Human Kinetochore Protein Architecture. eLife, 7, (2018).
-This article is recommended by “Faculty of 1000”.

2017

16) Mills, CA., Suzuki, A., Arceci, A., Mo, JY., Duncan, A., Salmon, ED., Emanuele M. Nucleolar and spindle-associated protein 1 (NUSAP1) interacts with a SUMO E3 ligase complex during chromosome segregation. Journal of Biological Chemistry, 292 (42), 17178-17189, (2017).
-Featured in cover.

15) Takada, M.†, Zhang, W.†, Suzuki, A., Kuroda, TS., Yu, Z., Inuzuka, H., Gao, D., Wan, L., Zhuang, M., Hu, L., Zhai, B., Fry, CJ., Bloom, K., Li, G., Karpen, GH.*, Wei, W.*, Zhang, Q.* FBW7 Loss Promotes Chromosomal Instability and Tumorigenesis via Cyclin E1/CDK2-Mediated Phosphorylation of CENP-A. Cancer Research, 77, 4881-4893, (2017).
-This article is recommended by “Faculty of 1000”.

2016

14) Lera, RF., Potts, GK., Suzuki, A., Johnson, JM., Salmon, ED., Coon, JJ., Burkard, ME. Decoding Polo-like kinase 1 signaling along the kinetochore-centromere axis. Nature Chemical Biology, 12, 411-418, (2016).

13) Suzuki, A.*, Badger, BL., Haase, J., Ohashi, T., Erickson, HP., Salmon ED., Bloom, K. How the kinetochore couples microtubule force and centromere stretch to move chromosomes. Nature Cell Biology, 18, 382-392, (2016).
-This article is recommended by “Faculty of 1000”.

12) Pilaz LJ., McMahon, JJ., Miller EE., Lennox, AL., Suzuki, A., Salmon, ED., Silver DL. Prolonged mitosis of neural progenitor alters cell fate in the developing brain. Neuron, 89(1), 83-99 (2016).
-This article is recommended by “Faculty of 1000”.

2015

11) Suzuki, A.*, Badger, BL., Salmon, ED.* A Quantitative Description of Ndc80 Complex Linkage to Human Kinetochore. Nature Communications, 6, 8161, (2015).

2014

10) Suzuki, A.*, Badger, BL., Wan, X., DeLuca, JG., Salmon, ED.* The architecture of CCAN proteins creates a structural integrity to resist spindle forces and achieve proper intrakinetochore stretch. Developmental Cell, 30, 717-730, (2014).
-Featured in Previews entitled “How Kinetochores CCAN Resist Force” in Developmental Cell, 30, 637-638.
-Featured on Developmental Cell’s top website page.

9) Ishiguro, KI., Kim, J., Shibuya H., Hernández-Hernández, A., Suzuki, A., Fukagawa, T., Shioi, G., Kiyonari, H., Li, XC., Schimenti, J., Höög, C., Watanabe Y. Meiosis-specific cohesion mediates homolog recognition in mouse spermatocytes. Genes & Development, 28, 594-607, (2014).

2013

8) Varma, D., Wan, X., Cheerambathur, D., Gassmann, R., Suzuki, A., Lawrimore, J., Desai, A., Salmon, ED. Spindle assembly checkpoint proteins are positioned close to core microtubule attachment sites at kinetochores. Journal of Cell Biology, 202, 735-746, (2013).

2012

7) Nishino, T., Takeuchi, K., Gascoigne, KE., Suzuki, A., Hori, T., Oyama, T., Morikawa, K., Cheeseman, IM., Fukagawa, T. CENP-T-W-S-X forms a unique centrometric chromatin structure with a histone-like fold. Cell, 148, 487-501, (2012).
-This article is recommended by “Faculty of 1000”.

2011

6) Gascoigne, KE., Takeuchi, K., Suzuki, A., Hori, T., Fukagawa, T., Cheeseman, IM. Induced Ectopic Kinetochore Assembly Bypasses the Requirement for CENP-A Nucleosomes. Cell, 145, 410-422, (2011).
-This article is recommended by “Faculty of 1000”.

5) Suzuki, A., Hori, T., Nishino, T., Usukura, J., Miyagi, A., Morikawa, K., Fukagawa, T. Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins. Journal of Cell Biology, 193, 125-140, (2011).
-Featured in In Focus entitled “Kinetochores feel the pull” in Journal of Cell Biology, 193, 3.
-This article is recommended by “Faculty of 1000”.

4) Suzuki, A., Fukagawa, T. Cell Biological Analysis of DT40 Knockout Cell Lines for Cell-Cycle Genes. Current Protocols in Cell Biology, 8; 8.7.1-8.7.17, (2011).

2010

3) Johnston, K., Joglekar, A., Hori, T., Suzuki, A., Fukagawa, T., Salmon, ED. Vertebrate kinetochore protein architecture: protein copy number. Journal of Cell Biology, 189(6), 937-943, (2010).

2009

2) Amano, M.#, Suzuki, A.#, Hori, T.#, Backer, C., Okawa, K., Cheeseman, IM., Fukagawa, T. The CENP-S complex is essential for the stable assembly of outer kinetochore structure. Journal of Cell Biology, 186, 173-182, (2009).

2008

1) Hori, T., Amano, M., Suzuki, A., Backer, CB., Welburn, JP., Dong, Y., McEwen, BF., Shang, WH., Suzuki, E., Okawa, K., Cheeseman, IM., Fukagawa, T. CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore. Cell, 135, 1039-1052, (2008).

Patent

Suzuki Lab is highlighted in WARF(Wisconsin Alumni Research Foundation) website

1. “KITS AND METHODS FOR SUPER-RESOLUTION MICROSCOPY”

Inventors: Aussie Suzuki, Mark Burkard, Roshan Norman, Emma Recchia

Patent Application Publication No. US-2022-0074829 and WARF: P200189US02

Highlighted in WARF microscopy special section

Licensed and commercialized kits will be available in Summer/Fall 2025

2. “Microtubule Labeled Slides Used for Calibration of Various Fluorescence Microscopes”

Inventors: Aussie Suzuki

WARF: P200284US01

Highlighted in WARF microscopy special section

3. “NOVEL CROSSLINKER FOR ALL EXPANSION MICROSCOPY”

Inventors: Aussie Suzuki

WARF: P250060US01